Staehlke, Susanne and Lehnfeld, Jutta and Schneider, Andreas and Nebe, J. Barbara and Mueller, Rainer (2019) Terminal chemical functions of polyamidoamine dendrimer surfaces and its impact on bone cell growth. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 101. pp. 190-203. ISSN 0928-4931, 1873-0191
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Besides their use for drug and gene delivery, dendrimer molecules are also favorable for the design of new surface coatings for orthopedic and dental implants due to the wide variety of functional terminal groups and their multivalent character. The purpose of this work was to observe how covalently immobilized polyamidoamine (PAMAM) dendrimer molecules with different terminal chemical groups influenced serum protein adsorption and osteoblast behavior. To this end, fifth-generation PAMAM dendrimers were immobilized on silicon surfaces with an anhydride-containing silane coupling agent which results in positively charged terminal NH2-groups. Coatings with a net negative charge were generated by introduction of terminal CO2H- or CH3- groups. Surface characterization was performed by static and dynamic contact angle and zeta potential. The in vitro studies with human MG-63 osteoblastic cells focused on cell adhesion, morphology, cell cycle, apoptosis and actin formation within 24 h. This work demonstrated that cell growth was dependent on surface chemistry and correlated strongly with the surface free energy and charge of the material. The positively charged NH 2 surface induced tight cell attachment with well-organized actin stress fibers and a well spread morphology. In contrast, CO2H- and CH3-functional groups provoked a decrease in cell adhesion and spreading and indicated higher apoptotic potential, although both were hydrophilic. The knowledge about the cell-material dialogue is of relevance for the development of bioactive implants in regenerative medicine.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SELF-ASSEMBLED MONOLAYERS; CONTACT-ANGLE HYSTERESIS; MESENCHYMAL STEM-CELLS; TITANIUM SURFACE; EUKARYOTIC CELLS; FOCAL ADHESION; IN-VITRO; CHEMISTRY; TOPOGRAPHY; ADSORPTION; Polyamidoamine dendrimer; Zeta-potential; Protein adsorption; MG-63 osteoblast; Cell cycle; Actin cytoskeleton |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Apr 2020 06:37 |
| Last Modified: | 07 Apr 2020 06:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26586 |
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