Farowski, Fedja and Solbach, Philipp and Tsakmaklis, Anastasia and Brodesser, Susanne and Aguilar, Rebeca Cruz and Cornely, Oliver A. and Dettmer, Katja and Higgins, Paul G. and Suerbaum, Sebastian and Jazmati, Nathalie and Oefner, Peter J. and Vehreschild, Maria J. G. T. (2019) Potential biomarkers to predict outcome of faecal microbiota transfer for recurrent Clostridioides difficile infection. DIGESTIVE AND LIVER DISEASE, 51 (7). pp. 944-951. ISSN 1590-8658, 1878-3562
Full text not available from this repository. (Request a copy)Abstract
Background & Aims: Faecal microbiota transplantation (FMT) has proven high clinical efficacy in the management of recurrent Clostridioides difficile infection (rCDI) with cure rates of over 80% after a single treatment. Nevertheless, the reasons for failure in the remaining 20% remain elusive. The aim of the present study was to investigate different potential predictors of response to FMT. Methods: Faecal specimens of sixteen patients undergoing FMT for rCDI, as well as samples from the respective donors were collected and analyzed by 16S rRNA gene profiling, bile acid-inducible (baiCD) gene specific qPCR, and liquid chromatography tandem-mass spectrometry (LC-MS/MS) to quantify the concentrations of primary and secondary bile acids. Results: Using the faecal concentration of the secondary bile acid lithocholic acid (LCA) within the patient specimens, we were able to predict response to FMT (accuracy 95.2%, sensitivity 100%, specificity 90.9%). By combining the faecal LCA concentration with the urinary pCS concentration, an accuracy of 100% was achieved. Conclusion: LCA appears to be a promising marker candidate for prediction of clinical response to FMT. Other makers, such as urinary concentration of pCS, but not 3-IS, might be used to improve accuracy of prediction. Further studies are warranted to validate these candidate markers. (C) 2019 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPORE GERMINATION; ACID; BILE; TRANSPLANTATION; ANTIBIOTICS; FIDAXOMICIN; VANCOMYCIN; BURDEN; Bile acids; Clostridioides difficile infection; Microbiota |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 06:58 |
| Last Modified: | 06 Apr 2020 06:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26779 |
Actions (login required)
 |
View Item |
