Laurentius, Thea and Raffetseder, Ute and Fellner, Claudia and Kob, Robert and Nourbakhsh, Mahtab and Floege, Juergen and Bertsch, Thomas and Bollheimer, Leo Cornelius and Ostendorf, Tammo (2019) High-fat diet-induced obesity causes an inflammatory microenvironment in the kidneys of aging Long-Evans rats. JOURNAL OF INFLAMMATION-LONDON, 16: 14. ISSN 1476-9255,
Full text not available from this repository. (Request a copy)Abstract
BackgroundObesity is a risk factor for chronic kidney disease (CKD). While the exact mechanisms remain unclear, inflammation may be a consequence of obesity that directly impacts the kidneys. The aim of this study was to examine the inflammatory status of the kidneys and potential ongoing renal damage, i.e., tubular damage and fibrosis after long-term obesity maintained through persistent consumption of a high-fat diet (HFD).ResultsTwenty-four-week-old male Long-Evans (LEV) rats were continuously fed a control diet (CD) or HFD for 51weeks. The mean body weight was higher in HFD-fed rats than in control diet-fed rats and markedly elevated during the last 24weeks. Blood analyses revealed no substantial alterations in renal functional parameters by HFD consumption but a substantial increase in creatine kinase, a muscle loss marker. Magnetic resonance imaging (MRI) was utilized to quantify rat quadriceps muscle mass. The data showed that HFD-induced obesity in LEV rats was accompanied by minor decreases in muscle mass and strength at 75weeks of age. Rat kidney inflammatory status was evaluated using histological and immunohistological techniques. The number of foci with immune cell infiltrates and infiltrating monocytes/macrophages was significantly increased in HFD-fed rat kidneys at week 75. Renal fibrosis parameters, including glomerulosclerosis and tubular damage, were also markedly increased in renal tissues from HFD-fed rats compared to the controls. The significant increase in tubular protein casts in HFD-fed rat tissues indicated that renal function was already disturbed. Rat kidney inflammatory status was further evaluated using the simultaneous profiling of twenty-two inflammatory markers in kidney tissue extracts. Consistently, MCP-1 and eotaxin (CCL11) levels were elevated in obese LEV rat kidneys.ConclusionsCompared to CD-fed rats, HFD-fed obese LEV rats show significant damage of renal structures with aging. These subtle changes may sensitize the kidneys to the development of progressive CKD.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RENAL INJURY; FIBROSIS; MUSCLE; BODY; ASSOCIATION; MODEL; MASS; Rat model; High-fat-diet; Obesity; Kidney; Inflammation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2020 06:03 |
| Last Modified: | 08 Apr 2020 06:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26810 |
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