Urdinguio, Rocio G. and Lopez, Virginia and Bayon, Gustavo F. and Diaz de la Guardia, Rafael and Sierra, Marta I. and Garcia-Torano, Estela and Perez, Raul F. and Garcia, Maria G. and Carella, Antonella and Pruneda, Patricia C. and Prieto, Cristina and Dmitrijeva, Marija and Santamarina, Pablo and Belmonte, Thalia and Mangas, Cristina and Diaconu, Elena and Ferrero, Cecilia and Ramon Tejedor, Juan and Luis Fernandez-Morera, Juan and Bravo, Cristina and Bueno, Clara and Sanjuan-Pla, Alejandra and Rodriguez, Ramon M. and Suarez-Alvarez, Beatriz and Lopez-Larrea, Carlos and Bernal, Teresa and Colado, Enrique and Balbin, Milagros and Garcia-Suarez, Olivia and Dolores Chiara, Maria and Saenz-de-Santa-Maria, Ines and Rodriguez, Francisco and Pando-Sandoval, Ana and Rodrigo, Luis and Santos, Laura and Salas, Ana and Vallejo-Diaz, Jesus and Carrera, Ana C. and Rico, Daniel and Hernandez-Lopez, Inmaculada and Vaya, Amparo and Ricart, Jose M. and Seto, Edward and Sima-Teruel, Nuria and Vaquero, Alejandro and Valledor, Luis and Jesus Canal, Maria and Pisano, David and Grana-Castro, Osvaldo and Thomas, Tim and Voss, Anne K. and Menendez, Pablo and Villar-Garea, Ana and Deutzmann, Rainer and Fernandez, Agustin F. and Fraga, Mario F. (2019) Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment. NUCLEIC ACIDS RESEARCH, 47 (10). pp. 5016-5037. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
Histone H4 acetylation at Lysine 16 (H4K16ac) is a key epigenetic mark involved in gene regulation, DNA repair and chromatin remodeling, and though it is known to be essential for embryonic development, its role during adult life is still poorly understood. Here we show that this lysine is massively hyperacetylated in peripheral neutrophils. Genome-wide mapping of H4K16ac in terminally differentiated blood cells, along with functional experiments, supported a role for this histone post-translational modification in the regulation of cell differentiation and apoptosis in the hematopoietic system. Furthermore, in neutrophils, H4K16ac was enriched at specific DNA repeats. These DNA regions presented an accessible chromatin conformation and were associated with the cleavage sites that generate the 50 kb DNA fragments during the first stages of programmed cell death. Our results thus suggest that H4K16ac plays a dual role in myeloid cells as it not only regulates differentiation and apoptosis, but it also exhibits a non-canonical structural role in poising chromatin for cleavage at an early stage of neutrophil cell death.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENSITY-DEPENDENT APOPTOSIS; H4K16 ACETYLATION; ACETYLTRANSFERASE HMOF; NEUTROPHIL APOPTOSIS; HEMATOPOIETIC STEM; DIMETHYL-SULFOXIDE; TOPOISOMERASE-I; GASTRIC-CANCER; MALES ABSENT; NG2 ANTIGEN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2020 13:40 |
| Last Modified: | 08 Apr 2020 13:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/26853 |
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