Schneider, Christin and Gebhardt, Lisa and Arndt, Stephanie and Karrer, Sigrid and Zimmermann, Julia L. and Fischer, Michael J. M. and Bosserhoff, Anja-Katrin (2019) Acidification is an Essential Process of Cold Atmospheric Plasma and Promotes the Anti-Cancer Effect on Malignant Melanoma Cells. CANCERS, 11 (5): 671. ISSN 2072-6694,
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(1) Background: Cold atmospheric plasma (CAP) is ionized gas near room temperature. The anti-cancer effects of CAP were confirmed for several cancer types and were attributed to CAP-induced reactive species. However, the mode of action of CAP is still not well understood. (2) Methods: Changes in cytoplasmic Ca2+ level after CAP treatment of malignant melanoma cells were analyzed via the intracellular Ca2+ indicator fura-2 AM. CAP-produced reactive species were determined by fluorescence spectroscopic and protein nitration by Western Blot analysis. (3) Results: CAP caused a strong acidification of water and solutions that were buffered with the so-called Good buffers, while phosphate-buffered solutions with higher buffer capacity showed minor pH reductions. The CAP-induced Ca2+ influx in melanoma cells was stronger in acidic pH than in physiological conditions. NO formation that is induced by CAP was dose- and pH-dependent and CAP-treated solutions only caused protein nitration in cells under acidic conditions. (4) Conclusions: We describe the impact of CAP-induced acidification on the anti-cancer effects of CAP. A synergistic effect of CAP-induced ROS, RNS, and acidic conditions affected the intracellular Ca2+ level of melanoma cells. As the microenvironment of tumors is often acidic, further acidification might be one reason for the specific anti-cancer effects of CAP.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MANGANESE-SUPEROXIDE-DISMUTASE; TYROSINE NITRATION; AQUEOUS-SOLUTIONS; PRESSURE PLASMA; NITRIC-OXIDE; INACTIVATION; ACID; AIR; PH; MICROENVIRONMENT; cold atmospheric plasma; malignant melanoma; calcium signaling; acidification; nitration |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Apr 2020 05:58 |
| Last Modified: | 15 Apr 2020 05:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27043 |
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