Hueynck, Jan Schulze and Kaschani, Farnusch and van der Linde, Karina and Ziemann, Sebastian and Mueller, Andre N. and Colby, Thomas and Kaiser, Markus and Villamil, Johana C. Misas and Doehlemann, Gunther (2019) Proteases Underground: Analysis of the Maize Root Apoplast Identifies Organ Specific Papain-Like Cysteine Protease Activity. FRONTIERS IN PLANT SCIENCE, 10: 473. ISSN 1664-462X,
Full text not available from this repository. (Request a copy)Abstract
Plant proteases are key regulators of plant cell processes such as seed development, immune responses, senescence and programmed cell death (PCD). Apoplastic papain-like cysteine proteases (PL) are hubs in plant-microbe interactions and play an important role during abiotic stresses. The apoplast is a crucial interface for the interaction between plant and microbes. So far, apoplastic maize PL and their function have been mostly described for aerial parts. In this study, we focused on apoplastic PLCPs in the roots of maize plants. We have analyzed the phylogeny of maize PLCPs and investigated their protein abundance after salicylic acid (SA) treatment. Using activity-based protein profiling (ABPP) we have identified a novel root-specific PLCP belonging to the RD21-like subfamily, as well as three SA activated PLCPs. The root specific PLCP CP1C shares sequence and structural similarities to known CP1-like proteases. Biochemical analysis of recombinant CP1C revealed different substrate specificities and inhibitor affinities compared to the related proteases. This study characterized a root-specific PLCP and identifies differences between the SA-dependent activation of PLCPs in roots and leaves.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CATHEPSIN-B; PHYTOPHTHORA-INFESTANS; C1A CYSTEINE; GENE FAMILY; CELL-DEATH; ARABIDOPSIS; INHIBITORS; SENESCENCE; CYSTATIN; PROTEOME; root; apoplast; PLCP; organ specific; salicylic acid |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Apr 2020 06:39 |
| Last Modified: | 15 Apr 2020 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27135 |
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