Grijalvo, Santiago and Puras, Gustavo and Zarate, Jon and Pons, Ramon and Luis Pedraz, Jose and Eritja, Ramon and Diaz Diaz, David (2016) Nioplexes encapsulated in supramolecular hybrid biohydrogels as versatile delivery platforms for nucleic acids. RSC ADVANCES, 6 (46). pp. 39688-39699. ISSN 2046-2069,
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Supramolecular hydrogels based on N-protected phenylalanine (Fmoc-Phe-OH) were used to encapsulate non-ionic surfactant vesicles (niosomes). The niosomes consisted of an amphiphilic lipid mixed with polysorbate-80 and electrostatically complexed with a fluorescently labelled oligodeoxynucleotide (FITC-ODN) as a model nucleic acid derivative. The diffusion properties of the supramolecular hydrogel were conveniently tuned by adding a small amount of kappa-carrageenan (<= 1% w/v) as a crosslinking agent. Interestingly, neither cationic niosomes nor the biopolymer additive significantly affected the hydrogelation properties of the amino acid-based low molecular weight (LMW) gelator. In vitro drug release experiments from Fmoc-Phe-OH hydrogels containing cationic niosomes were successfully carried out in the absence and in the presence of kappa-carrageenan. The niosomal ODN liberation in solution was fitted using Higuchi, Korsmeyer-Peppas and Weibull drug release models, showing the prevalence of diffusion mechanisms in each case. Moreover, the time release was easily prolonged by increasing the concentration of kappa-carrageenan. Preliminary transfection studies indicate the suitability of these supramolecular hybrid hydrogels to embed niosomal formulations and, consequently, for being used as tunable delivery vehicles for nucleic acids.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACTANT VESICLES NIOSOMES; DRUG-DELIVERY; NONIONIC SURFACTANT; CONTROLLED-RELEASE; ANTISENSE OLIGONUCLEOTIDES; SOLUTE RELEASE; HYDROGELS; LIPOSOMES; GELS; DERIVATIVES; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Prof. Dr. David Díaz Díaz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Mar 2019 10:18 |
| Last Modified: | 15 Mar 2019 10:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2724 |
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