Sosso, Davide and van der Linde, Karina and Bezrutczyk, Margaret and Schuler, David and Schneider, Karina and Kaemper, Joerg and Walbot, Virginia (2019) Sugar Partitioning between Ustilago maydis and Its Host Zea mays L during Infection. PLANT PHYSIOLOGY, 179 (4). pp. 1373-1385. ISSN 0032-0889, 1532-2548
Full text not available from this repository. (Request a copy)Abstract
The basidiomycete Ustilago maydis causes smut disease in maize (Zea mays) by infecting all plant aerial tissues. The infection causes leaf chlorosis and stimulates the plant to produce nutrient-rich niches (i.e. tumors), where the fungus can proliferate and complete its life cycle. Previous studies have recorded high accumulation of soluble sugars and starch within these tumors. Using interdisciplinary approaches, we found that the sugar accumulation within tumors coincided with the differential expression of plant sugars will eventually be exported transporters and the proton/sucrose symporter Sucrose Transporter1. To accumulate plant sugars, the fungus deploys its own set of sugar transporters, generating a sugar gradient within the fungal cytosol, recorded by expressing a cytosolic glucose (Glc) Forster resonance energy transfer sensor. Our measurements indicated likely elevated Glc levels in hyphal tips during infection. Growing infected plants under dark conditions led to decreased plant sugar levels and loss of the fungal tip Glc gradient, supporting a tight link between fungal sugar acquisition and host supplies. Finally, the fungal infection causes a strong imbalance in plant sugar distribution, ultimately impacting seed set and yield.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DISEASE-SUSCEPTIBILITY GENE; MONOSACCHARIDE TRANSPORTER; BACTERIAL-BLIGHT; H+/HEXOSE COTRANSPORTER; SUCROSE TRANSPORTER; SECRETED EFFECTOR; SMUT FUNGUS; ROOT HAIRS; MAIZE; ARABIDOPSIS; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Apr 2020 05:29 |
| Last Modified: | 15 Apr 2020 05:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27265 |
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