Fogelson, Susan B. and Camus, Alvin C. and Lorenz, W. Walter and Vasireddy, Ravikiran and Vasireddy, Sruthi and Smith, Terry and Brown-Elliott, Barbara A. and Wallace, Richard J. and Hasan, Nabeeh A. and Reischl, Udo and Sanchez, Susan (2019) Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns. PLOS ONE, 14 (3): e0214274. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Mycobacterium chelonae is a member of the Mycobacterium chelonae-abscessus complex and a cause of opportunistic disease in fish, reptiles, birds, and mammals including humans. Isolates in the complex are often difficult to identify and have differing antimicrobial susceptibilities. Thirty-one previously identified rapidly-growing, non-tuberculous Mycobacterium sp. isolates cultured from biofilms, fish, reptiles, mammals, including humans, and three ATCC reference strains were evaluated with nine M. chelonae-abscessus complex whole genome sequences from GenBank by phylogenomic analysis, targeted gene comparisons, and in-vitro antimicrobial susceptibility patterns to assess strain variation among isolates from different sources. Results revealed minimal genetic variation among the M. chelonae strains. However, the core genomic alignment and SNP pattern of the complete 16S rRNA sequence clearly separated the turtle type strain ATCC 35752(T) from the clinical isolates and human reference strain "M. chelonae chemovar niacinogenes" ATCC 19237, providing evidence of two distinct subspecies. Concatenation of the partial rpoB (752 bp) and complete hsp65 (1,626 bp) sequence produced the same species/subspecies delineations as the core phylogeny. Partial rpoB and hsp65 sequences identified all the clinical isolates to the appropriate species level when respective cut-offs of 98% and 98.4% identity to the M. chelonae type strain ATCC 35752(T) were employed. The human strain, ATCC19237, was the most representative strain for the evaluated human, veterinary, and environmental strains. Additionally, two isolates were identified as Mycobacterium saopaulense, its first identification in a non-fish or non-human host.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 16S RIBOSOMAL-RNA; NONTUBERCULOUS MYCOBACTERIA; SP NOV.; RAPID IDENTIFICATION; GROWING MYCOBACTERIA; SEQUENCE-ANALYSIS; INFECTIONS; AMPLIFICATION; DISEASE; HSP65; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Apr 2020 10:10 |
| Last Modified: | 15 Apr 2020 10:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27340 |
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