Maldonado, Rodrigo and Schwartz, Uwe and Silberhorn, Elisabeth and Laengst, Gernot (2019) Nucleosomes Stabilize ssRNA-dsDNA Triple Helices in Human Cells. MOLECULAR CELL, 73 (6). 1243-+. ISSN 1097-2765, 1097-4164
Full text not available from this repository. (Request a copy)Abstract
Chromatin-associated non-coding RNAs modulate the epigenetic landscape and its associated gene expression program. The formation of triple helices is one mechanism of sequence-specific targeting of RNA to chromatin. With this study, we show an important role of the nucleosome and its relative positioning to the triplex targeting site (TTS) in stabilizing RNA-DNA triplexes in vitro and in vivo. Triplex stabilization depends on the histone H3 tail and the location of the TTS close to the nucleosomal DNA entry-exit site. Genome-wide analysis of TTS-nucleosome arrangements revealed a defined chromatin organization with an enrichment of arrangements that allow triplex formation at active regulatory sites and accessible chromatin. We further developed a method to monitor nucleosome-RNA triplexes in vivo (TRIP-seq), revealing RNA binding to TTS sites adjacent to nucleosomes. Our data strongly support an activating role for RNA triplex-nucleosome complexes, pinpointing triplex-mediated epigenetic regulation in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OLIGONUCLEOTIDE TARGET SEQUENCES; HISTONE TAIL DOMAINS; CORE PARTICLE; THIAZOLE ORANGE; NONCODING RNA; DNA; GENE; SIGNATURES; ELEMENTS; CLEAVAGE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Apr 2020 10:09 |
| Last Modified: | 15 Apr 2020 10:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27346 |
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