Fritsch, Juergen and Tchikov, Vladimir and Hennig, Lena and Lucius, Ralph and Schuetze, Stefan (2019) A toolbox for the immunomagnetic purification of signaling organelles. TRAFFIC, 20 (3). pp. 246-258. ISSN 1398-9219, 1600-0854
Full text not available from this repository. (Request a copy)Abstract
Homeostasis and the complex functions of organisms and cells rely on the sophisticated spatial and temporal regulation of signaling in different intra- and extracellular compartments and via different mediators. We here present a set of fast and easy to use protocols for the target-specific immunomagnetic enrichment of receptor containing endosomes (receptosomes), plasma membranes, lysosomes and exosomes. Isolation of subcellular organelles and exosomes is prerequisite for and will advance their detailed subsequent biochemical and functional analysis. Sequential application of the different subprotocols allows isolation of morphological and functional intact organelles from one pool of cells. The enrichment is based on a selective labelling using receptor ligands or antibodies together with superparamagnetic microbeads followed by separation in a patented matrix-free high-gradient magnetic purification device. This unique magnetic chamber is based on a focusing system outside of the empty separation column, generating an up to 3 T high-gradient magnetic field focused at the wall of the column.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TNF RECEPTOR 1; NF-KAPPA-B; ACID SPHINGOMYELINASE; LIPID RAFTS; UBIQUITINATION; ENDOCYTOSIS; INTERNALIZATION; PROTEOLYSIS; TRAFFICKING; ACTIVATION; cell death; cell fractionation; cytokine receptors; organelles; purification; signal transduction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Apr 2020 10:11 |
| Last Modified: | 21 Apr 2020 10:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27496 |
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