Durner, Bernhard and Ehmann, Thomas and Matysik, Frank-Michael (2019) High-resolution polymer high performance liquid chromatography: Application of a saw tooth gradient for the separation of various polymers. JOURNAL OF CHROMATOGRAPHY A, 1587. pp. 88-100. ISSN 0021-9673, 1873-3778
Full text not available from this repository. (Request a copy)Abstract
Currently, a lot of research effort in polymer analysis by liquid chromatographic techniques, including size exclusion chromatography (SEC), polymer HPLC or liquid chromatography at critical conditions, is done aiming to improve separation performance. In this study, novel gradient protocols were investigated primarily based on gradient polymer elution chromatography (GPEC). Starting with linear gradients and stepwise gradients a new periodic saw tooth gradient profile was developed and optimized. Optimum settings for the saw tooth gradient design were evaluated by design of experiments (DoE) based on Taguchi's methodology for various types of stationary phases. The gain of peak resolution was dependent on the effective gradient step height. The optimized protocol enabled high-resolution polymer HPLC (HRP-HPLC) separations with common HPLC instruments. The quality of separation was evaluated by heart-cut fraction collection of HRP-HPLC and subsequent determination of the individual fractions by SEC or MALDI-ToF mass spectrometry. Finally, different types of polymers, such as PVC, PDMS, PMMA, or PPG, were studied with the new method and a universal applicability was shown. (C) 2018 Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MOLAR-MASS POLYMERS; HUMIC-LIKE SUBSTANCES; ELUTION CHROMATOGRAPHY; POLYESTER RESINS; PHASE; OPTIMIZATION; FRACTIONATION; BEHAVIOR; High-resolution polymer high performance; liquid chromatography (HRP-HPLC); Saw tooth gradient; Polystyrene (PS); Poly(vinylchloride) (PVC); Poly(methylmethacrylate) (PMMA); Poly(dimethylsiloxane) (PDMS) |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Apr 2020 06:30 |
| Last Modified: | 17 Apr 2020 06:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27526 |
Actions (login required)
 |
View Item |
