Fiedler, Isabell-Christin and Weiberg, Arne and van der Linde, Karina (2019) Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (144): e58746. ISSN 1940-087X,
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Inspired by Homer's Trojan horse myth, we engineered the maize pathogen Ustilago maydis to deliver secreted proteins into the maize apoplast permitting in vivo phenotypic analysis. This method does not rely on maize transformation but exploits microbial genetics and secretory capabilities of pathogens. Herein, it allows inspection of in vivo delivered secreted proteins with high spatiotemporal resolution at different kinds of infection sites and tissues. The Trojan horse strategy can be utilized to transiently complement maize loss-of-function phenotypes, to functionally characterize protein domains, to analyze off-target protein effects, or to study onside protein overdosage, making it a powerful tool for protein studies in the maize crop system. This work contains a precise protocol on how to generate a Trojan horse strain followed by standardized infection protocols to apply this method to three different maize tissue types.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SECRETED EFFECTOR; Genetics; Issue 144; Ustilago maydis; Trojan Horse; Protein Delivery; Zea mays; Tassel; Anther; Ear; Cob; Silk; Adult Leaf |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Apr 2020 12:17 |
| Last Modified: | 17 Apr 2020 12:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27585 |
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