Muschter, Dominique and Beiderbeck, Anna-Sophie and Spaeth, Tanja and Kirschneck, Christian and Schroeder, Agnes and Graessel, Susanne (2019) Sensory Neuropeptides and their Receptors Participate in Mechano-Regulation of Murine Macrophages. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20 (3): 503. ISSN 1422-0067,
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This study aimed to analyze if the sensory neuropeptide SP (SP) and the neurokinin receptor 1 (NK1R) are involved in macrophage mechano-transduction, similar to chondrocytes, and if alpha-calcitonin gene-related peptide (CGRP) and the CGRP receptor (CRLR/Ramp1) show comparable activity. Murine RAW264.7 macrophages were subjected to a cyclic stretch for 1-3 days and 4 h/day. Loading and neuropeptide effects were analyzed for gene and protein expression of neuropeptides and their receptors, adhesion, apoptosis, proliferation and ROS activity. Murine bone marrow-derived macrophages (BMM) were isolated after surgical osteoarthritis (OA) induction and proliferation, apoptosis and osteoclastogenesis were analyzed in response to loading. Loading induced NK1R and CRLR/Ramp1 gene expression and altered protein expression in RAW264.7 macrophages. SP protein and mRNA level decreased after loading whereas CGRP mRNA expression was stabilized. SP reduced adhesion in loaded RAW264.7 macrophages and both neuropeptides initially increased the ROS activity followed by a time-dependent suppression. OA induction sensitized BMM to caspase 3/7 mediated apoptosis after loading. Both sensory neuropeptides, SP and CGRP, and their receptors are involved in murine macrophage mechano-transduction affecting neuropeptide impact on adhesion and ROS activity. OA induction altered BMM apoptosis in response to loading indicate that OA-associated biomechanical alterations might affect the macrophage population.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARROW-DERIVED MACROPHAGES; SUBSTANCE-P; NEUROKININ-1 RECEPTOR; EXTRACELLULAR-MATRIX; GENE-EXPRESSION; CGRP RECEPTORS; BONE; PROMOTES; OSTEOCLASTOGENESIS; OSTEOARTHRITIS; cyclic stretch; mechan-oregulation; murine macrophages; substance P; alpha-calcitonin gene-related peptide; neurokinin receptor; CGRP receptor; destabilized medial meniscus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Apr 2020 06:20 |
| Last Modified: | 22 Apr 2020 06:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27588 |
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