Schroeder, Agnes and Nazet, Ute and Muschter, Dominique and Graessel, Susanne and Proff, Peter and Kirschneck, Christian (2019) Impact of Mechanical Load on the Expression Profile of Synovial Fibroblasts from Patients with and without Osteoarthritis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20 (3): 585. ISSN 1422-0067,
Full text not available from this repository. (Request a copy)Abstract
Osteoarthritis (OA) affects the integrity of the entire joint including the synovium. The most abundant cells in the synovium are fibroblasts (SF). Excessive mechanical loading might contribute to OA pathogenesis. Here, we investigate the effects of mechanical loading on SF derived from non-OA (N-SF) and OA patients (OA-SF). We treated N-SF and OA-SF with or without mechanical loading for 48h after 24h of preincubation. Then we assessed gene and protein expression of proinflammatory factors (TNF, COX-2, PG-E2, IL-6), extracellular matrix (ECM) components (COL1, FN1) and glycosaminoglycans (GAGs) via RT-qPCR, ELISA, DMMB assay and HPLC. Mechanical loading significantly increased TNF and PG-E2 secretion by N-SF and OA-SF, whereas in OA-SF IL-6 secretion was reduced. COL1 and FN1 secretion were downregulated in N-SF during loading. OA-SF secreted less COL1 compared to N-SF under control conditions. In contrast, OA-SF in general expressed more FN1. GAG synthesis was upregulated in N-SF, but not in OA-SF during loading with OA-SF displaying a higher charge density than N-SF. Mechanical loading enhanced proinflammatory factor expression and GAG synthesis and decreased secretion of ECM components in N-SFs, indicating a contributing role of SF to OA development.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SERUM HYALURONIC-ACID; RHEUMATOID-ARTHRITIS; PROTEOGLYCAN DEGRADATION; ARTICULAR CHONDROCYTES; GENE-EXPRESSION; TNF-ALPHA; CARTILAGE; JOINT; INFLAMMATION; RECEPTOR; osteoarthritis; synovial fibroblasts; mechanical loading; synovium; inflammation; synovitis; compressive force |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Apr 2020 06:47 |
| Last Modified: | 22 Apr 2020 06:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27590 |
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