Kratzer, Alexander and Schiesser, Selina and Matzneller, Peter and Wulkersdorfer, Beatrix and Zeitlinger, Markus and Schlossmann, Jens and Kees, Frieder and Dorn, Christoph (2019) Determination of total and free ceftolozane and tazobactam in human plasma and interstitial fluid by HPLC-UV. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 163. pp. 34-38. ISSN 0731-7085, 1873-264X
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Ceftolozane/tazobactam is a new cephalosporin/beta-lactamase inhibitor combination. An HPLC-UV method is described for the determination of total and free ceftolozane and tazobactam in human plasma and in microdialysate of subcutaneous tissue, respectively. Separation was performed using a reversed-phase column with phosphate buffer/acetonitrile as eluent and photometric detection at 260 nm (ceftolozane) or 220 nm (tazobactam). Linearity has been shown down to ceftolozane/tazobactam 0.1/0.05 mg/L in plasma and 0.03/0.015 mg/L in saline, respectively. The plasma protein binding of both drugs as determined by ultrafiltration was less than 10%. Temperature, pH or relative centrifugation force (up to 3000 x g) had no significant impact on the protein binding. The method was applied to the determination of ceftolozane and tazobactam in plasma and interstitial fluid of healthy volunteers following intravenous infusion of ceftolozane/tazobactam 1.0/0.5 g. (C) 2018 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-BINDING; PHARMACOKINETICS; SAFETY; CEFTRIAXONE; VANCOMYCIN; CEFAZOLIN; PRESSURE; SINGLE; SERUM; VIVO; Cephalosporin; Beta-Lactamase inhibitor; Plasma protein binding; Tissue; Ultrafiltration; Microdialysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Chemistry and Pharmacy > Institute of Pharmacy Chemistry and Pharmacy > Institute of Pharmacy > Pharmacology and Toxicology (Prof. Schlossmann, formerly Prof. Seifert) Chemistry and Pharmacy > Institute of Pharmacy > Group Clinical Pharmacy (Dr. Dorn) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Apr 2020 08:46 |
| Last Modified: | 20 Apr 2020 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27695 |
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