Semmelmann, Florian and Hofferberth, John and Ruther, Joachim and Sterner, Reinhard (2019) Mapping key amino acid residues for the epimerase efficiency and stereospecificity of the sex pheromone biosynthetic short-chain dehydrogenases/reductases of Nasonia. SCIENTIFIC REPORTS, 9: 330. ISSN 2045-2322,
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Males of the parasitic wasp genus Nasonia use blends of chiral hydroxylactones as sex pheromones to attract conspecific females. Whereas all Nasonia species use a mixture of (4R,5S)-5-hydroxy-4-decanolide (RS) and 4-methylquinazoline (MQ) as sex pheromones, Nasonia vitripennis evolved (4R, 5R)-5-hydroxy-4-decanolide (RR) as an extra sex pheromone component. We recently identified and functionally characterized three short-chain dehydrogenases/ reductases (SDRs) NV10127, NV10128, and NV10129 that are capable of catalyzing the epimerization of RS to RR via (4R)-5-oxo-4-decanolide (ODL) as intermediate. Despite their very high sequence identities of 88-98%, these proteins differ drastically in their ability to epimerize RS to RR and in their stereoselectivity when reducing ODL to RR/RS. Here, in order to unravel the sequence differences underlying these varying functional properties of NV1027, NV10128 and NV10129, we created chimeras of the three enzymes and monitored their catalytic activities in vitro. The results show that a few amino acid changes at the C-termini and active sites of Nasonia vitripennis SDRs lead to substantially altered RS to RR epimerization and ODLreduction activities. Thus, our study adds to the understanding of pheromone evolution by showing that subtle mutations in key biosynthetic enzymes can result in drastic effects on the composition of chemical signals.
Item Type: | Article |
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Uncontrolled Keywords: | UDP-GALACTOSE 4-EPIMERASE; EVOLUTION; PTEROMALIDAE; SPECIFICITY; |
Subjects: | 500 Science > 570 Life sciences 500 Science > 590 Zoological sciences |
Divisions: | Biology, Preclinical Medicine > Institut für Zoologie Biology, Preclinical Medicine > Institut für Zoologie > Chemische Ökologie (Prof. Dr. Joachim Ruther) Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner |
Depositing User: | Dr. Gernot Deinzer |
Date Deposited: | 20 Apr 2020 09:12 |
Last Modified: | 20 Apr 2020 09:12 |
URI: | https://pred.uni-regensburg.de/id/eprint/27707 |
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