Gustafsson, Charlotte and De Paepe, Ayla and Schmidl, Christian and Mansson, Robert (2019) High-throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers. BMC GENOMICS, 20: 59. ISSN 1471-2164,
Full text not available from this repository. (Request a copy)Abstract
BackgroundChromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level.ResultsWe present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes.ConclusionsThe resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHROMATIN; SEQ; ENHANCERS; Chromatin immunoprecipitation; ChIP-seq; ChIPmentation; High-throughput genomics; Epigenetics |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Apr 2020 07:14 |
| Last Modified: | 27 Apr 2020 07:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27718 |
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