Hlavacek, Antonin and Mickert, Matthias J. and Soukka, Tero and Lahtinen, Satu and Tallgren, Terhi and Pizurova, Nadezda and Krol, Anna and Gorris, Hans H. (2019) Large-Scale Purification of Photon-Upconversion Nanoparticles by Gel Electrophoresis for Analogue and Digital Bioassays. ANALYTICAL CHEMISTRY, 91 (2). pp. 1241-1246. ISSN 0003-2700, 1520-6882
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The performance of photon-upconversion nanoparticles (UCNPs) as background-free luminescent labels in bioanalytical applications strongly depends on the preparation of well-defined and water-dispersible nano conjugates. We have exploited the separation power of agarose-gel electrophoresis to purify milligram amounts of homogeneous UCNPs covered with carboxylated silica, biotin, or streptavidin with recovery rates of 30 to 50%. Clusters containing discrete numbers of UCNPs were isolated from the gel and reanalyzed by agarose-gel electrophoresis, single-nanoparticle-upconversion microscopy, and additional complementary methods. The purified nanoconjugates improved conventional (analogue) bioaffinity assays and provided highly monodisperse conjugates for assays that rely on counting individual UCNPs (digital assays).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LINKED IMMUNOSORBENT-ASSAY; UPCONVERTING NANOPARTICLES; SENSITIVE DETECTION; GOLD NANOPARTICLES; OPTICAL-PROPERTIES; SEPARATION; SIZE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Apr 2020 08:14 |
| Last Modified: | 27 Apr 2020 08:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/27721 |
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