Hlavacek, Antonin and Farka, Zdenek and Huebner, Maria and Hornakova, Veronika and Nemecek, Daniel and Niessner, Reinhard and Skladal, Petr and Knopp, Dietmar and Gorris, Hans H. (2016) Competitive Upconversion-Linked Immunosorbent Assay for the Sensitive Detection of Diclofenac. ANALYTICAL CHEMISTRY, 88 (11). pp. 6011-6017. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Photon-upconverting nanoparticles (UCNPs) emit light of shorter wavelength under near-infrared excitation and thus avoid optical background interference. We have exploited this unique photophysical feature to establish a sensitive competitive immunoassay for the detection of the pharmaceutical micropollutant diclofenac (DCF) in water. The so-called upconversion-linked immunosorbent assay (ULISA) was critically dependent on the design of the upconversion luminescent detection label. Silica coated UCNPs (50 nm in diameter) exposing carboxyl groups on the surface were conjugated to a secondary anti-IgG antibody. We investigated the structure and monodispersity of the nanoconjugates in detail. Using a highly affine anti-DCF primary antibody, the optimized ULISA reached a detection limit of 0.05 ng DCF per mL. This performance came close to a conventional enzyme linked immunosorbent assay (ELISA) without the need for an enzyme-mediated signal amplification step. The ULISA was further employed for analyzing drinking and surface water samples. The results were consistent with a conventional ELISA as well as liquid chromatography mass spectrometry (LC-MS).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTON-UPCONVERTING NANOPARTICLES; PHARMACEUTICAL RESIDUES; ENVIRONMENTAL-SAMPLES; SILICA NANOPARTICLES; SURFACE MODIFICATION; PHOSPHOR TECHNOLOGY; AQUATIC ENVIRONMENT; MASS-SPECTROMETRY; WHOLE-BLOOD; WATER; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2019 08:49 |
| Last Modified: | 18 Mar 2019 08:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2780 |
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