Bidmanova, Sarka and Steiner, Mark-Steven and Stepen, Martin and Vymazalova, Kamila and Gruber, Michael A. and Duerkop, Axel and Damborsky, Jiri and Prokop, Zbynek and Wolfbeis, Otto S. (2016) Enzyme-Based Test Strips for Visual or Photographic Detection and Quantitation of Gaseous Sulfur Mustard. ANALYTICAL CHEMISTRY, 88 (11). pp. 6044-6049. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Sulfur mustard is a chemical agent of high military and terroristic significance. No effective antidote exists, and sulfur mustard can be fairly easily produced in large quantity. Rapid field testing of sulfur mustard is highly desirable. Existing analytical devices for its detection are available but can suffer from low selectivity, laborious sample preparation, and/or the need for complex instrumentation. We describe a new kind of test strip for rapid detection of gaseous sulfur mustard that is based on its degradation by the enzyme haloalkane dehalogenase that is accompanied by a change of local pH. This change can be detected using pH indicators contained in the strips whose color changes from blue-green to yellow within 10 min. In addition to visual read-out, we also demonstrate quantitative reflectometric readout by using a conventional digital camera based on red-green-blue data acquisition. Organic haloalkanes, such as 1,2-dichloroethane, have a negligible interfering effect. The visual limit of detection is 20 mu g/L, and the one for red-green-blue read-out is as low as 3 mu g/L. The assays have good reproducibility +/- 6% and +/- 2% for interday assays and intraday assays, respectively. The strips can be stored for at least 6 months without loss of function. They are disposable and can be produced fairly rapidly and at low costs. Hence, they represent a promising tool for in-field detection of sulfur mustard.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PH; OXYGEN; TEMPERATURE; SMARTPHONE; SENSOR; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Mar 2019 08:58 |
| Last Modified: | 18 Mar 2019 08:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2781 |
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