Konz, Dirk D. and Fluegel-Koch, Cassandra and Ohlmann, Andreas and Tamm, Ernst R. (2009) Myocilin in the trabecular meshwork of eyes with primary open-angle glaucoma. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 247 (12). pp. 1643-1649. ISSN 0721-832X, 1435-702X
Full text not available from this repository. (Request a copy)Abstract
Mutations in myocilin, a 55-57 kDa secreted glycoprotein, are causative for some forms of primary open-angle glaucoma (POAG). In vitro studies indicate that myocilin can modulate the hydrodynamic outflow resistance in the trabecular meshwork (TM) and that elevated amounts of myocilin can obstruct the TM outflow system in POAG. In this study, we analyzed the localization of myocilin in the trabecular meshwork (TM) of eyes with primary open-angle glaucoma (POAG), and compared it with that of normal eyes. Immunohistochemistry for myocilin was performed in the eyes of human donors (nine normal and 14 with POAG, including one with steroid-induced glaucoma). Staining for myocilin was observed in the extracellular spaces of the juxtacanalicular tissue (JCT) in all normal eyes. Some normal eyes did also show cytoplasmic staining for myocilin in TM cells. In the eyes of six donors with POAG, staining of the JCT was more widespread and intense than in normal eyes. In the other eyes with POAG, immunoreactivity for myocilin in the JCT was not markedly different to that of normal eyes. Staining intensity in the JCT of POAG eyes did not obviously correlate with intraocular pressure or clinical severity. In the eyes of one patient with steroid-induced POAG, cells of the TM, Schlemm's canal endothelium, and the anterior stroma of the iris showed an immunoreactivity for myocilin which was considerably more intense than in normal eyes, or in the eyes with other forms of POAG. In some cases of POAG, the structural changes in the JCT include an increase in myocilin in the extracellular pathways of aqueous humor. Treatment with steroids appears to increase myocilin synthesis in TM and iris of human eyes in situ.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLUCOCORTICOID RESPONSE PROTEIN; INCREASES OUTFLOW RESISTANCE; AQUEOUS-HUMOR; EXTRACELLULAR-MATRIX; GENE-EXPRESSION; TRANSGENIC MICE; IN-VITRO; LOCALIZATION; FIBRONECTIN; INDUCTION; Aqueous humor outflow; Myocilin; Trabecular meshwork |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie > Prof. Dr. Ernst Tamm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Aug 2020 06:50 |
| Last Modified: | 31 Aug 2020 06:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28033 |
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