Steiner, Mark-Steven and Meier, Robert J. and Spangler, Christian and Duerkop, Axel and Wolfbeis, Otto S. (2009) Determination of biogenic amines by capillary electrophoresis using a chameleon type of fluorescent stain. MICROCHIMICA ACTA, 167 (3-4). pp. 259-266. ISSN 0026-3672, 1436-5073
Full text not available from this repository. (Request a copy)Abstract
A method was developed for the determination of biogenic amines (BAs) via micellar electrokinetic chromatography along with laser induced fluorescence detection using the amino-reactive chameleon stain Py-1. A labeling protocol was established for seven primary BAs by optimizing the reaction conditions in terms of the amount of reagents, reaction temperature, reaction time and solvent. Derivatization was accomplished within 30 min and is visible by the naked eye because it is accompanied by a color change from blue to red. Separation of the labeled BAs was achieved within 15 min with a background buffer of pH 2.5 containing phosphate, TweenA (R) 80, and methanol. The LODs range from 0.1 to 0.9 A mu mol center dot L-1, with RSDs ranging from 1.1 to 4.2% at 10 A mu mol center dot L-1. The method was applied to the determination of histamine in various fish samples.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ON-COLUMN DERIVATIZATION; ALIPHATIC-AMINES; CHROMATOGRAPHY; PROTEINS; SAMPLES; FOODS; LABELS; Biogenic amines; Capillary electrophoresis; Chameleon label; Derivatization; Laser-induced fluorescence |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Aug 2020 09:33 |
| Last Modified: | 31 Aug 2020 09:33 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28067 |
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