Blume, Annegret and Torner, Luz and Liu, Ying and Subburaju, Sivan and Aguilera, Greti and Neumann, Inga D. (2009) Prolactin induces Egr-1 gene expression in cultured hypothalamic cells and in the rat hypothalamus. BRAIN RESEARCH, 1302. pp. 34-41. ISSN 0006-8993, 1872-6240
Full text not available from this repository. (Request a copy)Abstract
Prolactin (PRL), the major lactogenic hormone, acts also as neuromodulator and regulator of neuronal and glial plasticity in the brain. There is an increase in synthesis and release of PRL within the hypothalamus during peripartum and in response to stress. To identify mechanisms by which PRL induces neuroplasticity, we studied the ability of PRL to induce the transcription factor Egr-1 in the hypothalamic cell line, 4B, in vitro, and in specific neuronal cell types of the hypothalamus in vivo. PAL induced Egr-1 mRNA expression in 4B cells, an effect which was prevented by the MEK inhibitor, U0126. In vivo, intracerebroventricular PRL (1 mu g) increased Egr-1 mRNA levels in the hypothalamic paraventricular (PVN) and supraoptic nuclei (SON) of female rats. The increase in mRNA paralleled elevated Egr-1 protein expression in the PVN and SON. Double staining immunohistochemistry revealed Egr-1 localization in oxytocin neurons of the PVN and SON, but not in vasopressin neurons in these regions. In the dorsomedial PVN, a population of non-oxytocin or vasopressin cells localized in a region corresponding to corticotropin-releasing hormone neurons also showed marked Egr-1 immunoreactivity. The data suggest that PRL modulates plasticity in oxytocinergic neurons, through MAP kinase-dependent induction of Egr-1. Published by Elsevier B.V.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TRANSCRIPTION FACTOR ZIF268/EGR-1; CORTICOTROPIN-RELEASING HORMONE; IMMEDIATE-EARLY GENE; NERVOUS-SYSTEM; MESSENGER-RNA; HYPOTHALAMONEUROHYPOPHYSEAL SYSTEM; STIMULATED NEUROGENESIS; GROWTH-FACTOR; LONG FORM; RECEPTOR; Signaling; Neuroplasticity; Stress; Hypothalamo-pituitary-adrenal axis; Transcription factor; Lactation |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Tierphysiologie/Neurobiologie (Prof. Dr. Inga Neumann) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Sep 2020 05:09 |
| Last Modified: | 02 Sep 2020 05:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28126 |
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