Kostrewa, Dirk and Zeller, Mirijam E. and Armache, Karim-Jean and Seizl, Martin and Leike, Kristin and Thomm, Michael and Cramer, Patrick (2009) RNA polymerase II-TFIIB structure and mechanism of transcription initiation. NATURE, 462 (7271). pp. 323-330. ISSN 0028-0836, 1476-4687
Full text not available from this repository. (Request a copy)Abstract
To initiate gene transcription, RNA polymerase II (Pol II) requires the transcription factor IIB (B). Here we present the crystal structure of the complete Pol II-B complex at 4.3 angstrom resolution, and complementary functional data. The results indicate the mechanism of transcription initiation, including the transition to RNA elongation. Promoter DNA is positioned over the Pol II active centre cleft with the 'B-core' domain that binds the wall at the end of the cleft. DNA is then opened with the help of the 'B-linker' that binds the Pol II rudder and clamp coiled-coil at the edge of the cleft. The DNA template strand slips into the cleft and is scanned for the transcription start site with the help of the 'B-reader' that approaches the active site. Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | START SITE SELECTION; N-TERMINAL REGION; PREINITIATION COMPLEX; ANGSTROM RESOLUTION; ELONGATION COMPLEX; CRYSTAL-STRUCTURE; SACCHAROMYCES-CEREVISIAE; ZINC RIBBON; YEAST; DNA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Sep 2020 05:23 |
| Last Modified: | 02 Sep 2020 05:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28129 |
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