Angele, Peter and Mueller, Rainer and Schumann, Detlef and Englert, Carsten and Zellner, Johannes and Johnstone, Brian and Yoo, Jung and Hammer, Joachim and Fierlbeck, Johann and Angele, Martin K. and Nerlich, Michael and Kujat, Richard (2009) Characterization of esterified hyaluronan-gelatin polymer composites suitable for chondrogenic differentiation of mesenchymal stem cells. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 91A (2). pp. 416-427. ISSN 1549-3296, 1552-4965
Full text not available from this repository. (Request a copy)Abstract
Composite scaffolds of homogeneously mixed esterified hyaluronan (HY) and gelatin (G) were manufactured with variable component compositions (HY100%; HY95%/G5%; HY70%/G30%). The goals of this study, were to analyze the produced composite scaffolds using physical and chemical methods, for example, scanning electron microscopy, IR-spectroscopy, water contact angle, protein assay, and tensile testing as well as to assess the effects of adding gelatin to the composite scaffolds on attachment, proliferation, and chondrogenic differentiation of human mesenchymal stem cells. Numbers of attached cells were significantly higher on the composite material compared to pure hyaluronan at different time points of two-dimensional or three-dimensional cell culture (p < 0.02). In composite scaffolds, a significantly greater amount of cartilage-specific extracellular matrix components was deposited after 28 days in culture (glycosaminoglycan: p < 0.001; collagen: p < 0.001) as compared with 100% hyaluronan scaffolds. Additionally, gelatin-containing composite scaffolds displayed stronger promotion of collagen type II expression than pure hyaluronan scaffolds. The mechanism, based on which gelatin influences cell adhesion, was examined. The effect was inhibited by collagenase treatment of the composites or by addition of alpha 5 beta 1-integrin blocking antibodies to the cell suspension. In summary, the results describe the establishment of a class of composite polymer scaffolds, consisting of esterified hyaluronan and gelatin, which are potentially useful for cell-based tissue engineering approaches using mesenchymal stem cells for chondrogenic differentiation. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91 A: 416-427, 2009
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TISSUE ENGINEERING SCAFFOLDS; IN-VITRO CHONDROGENESIS; BONE-MARROW; PROGENITOR CELLS; CROSS-LINKING; EXTRACELLULAR-MATRIX; PHYSICOCHEMICAL PROPERTIES; OSTEOCHONDRAL DEFECTS; BIOLOGICAL STABILITY; ACID HYDROGELS; gelatin; hyaluronan; tissue engineering; chondrogenesis; mechanical properties; mesenchymal stem cells |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > PD Dr. Rainer Müller |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Sep 2020 09:36 |
| Last Modified: | 02 Sep 2020 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28203 |
Actions (login required)
 |
View Item |
