Appel, B. and Baumer, J. and Eyrich, D. and Sarhan, H. and Toso, S. and Englert, C. and Skodacek, D. and Ratzinger, S. and Graessel, S. and Goepferich, A. and Blunk, Torsten (2009) Synergistic effects of growth and differentiation factor-5 (GDF-5) and insulin on expanded chondrocytes in a 3-D environment. OSTEOARTHRITIS AND CARTILAGE, 17 (11). pp. 1503-1512. ISSN 1063-4584, 1522-9653
Full text not available from this repository. (Request a copy)Abstract
Objective: To investigate the effects of growth and differentiation factor-5 (GDF-5) alone or in combination with insulin on engineered cartilage from primary or expanded chondrocytes during 3-dimensional in vitro culture. Design: Juvenile bovine chondrocytes were seeded either as primary or as expanded (passage 2) cells onto polyglycolic acid fiber meshes and cultured for 3 weeks in vitro. Additionally, adult human chondrocytes were grown in pellet culture after expansion (passage 2). The culture medium was supplemented either with GDF-5 in varying concentrations or insulin alone, or with combinations thereof. Results: For primary chondrocytes, the combination of GDF-5 and insulin led to increased proliferation and construct weight, as compared to either factor alone, however, the production of glycosaminoglycans (GAG) and collagen per cell were not affected. With expanded bovine chondrocytes, the use of GDF-5 or insulin alone led to only very small constructs with no type II collagen detectable. However, the combination of GDF-5 (0.01 or 0.1 mu g/ml) and insulin (2.5 mu g/ml) yielded cartilaginous constructs and, in contrast to the primary cells, the observed redifferentiating effects were elicited on the cellular level independent of proliferation (increased production of GAG and collagen per cell, clear shift in collagen subtype expression with type II collagen observed throughout the construct). The synergistic redifferentiating effects of the GDF-5/insulin combination were confirmed with expanded adult human cells, also exhibiting a clear shift in collagen subtype expression on the mRNA and protein level. Conclusions: In combination with insulin, GDF-5 appears to enable the redifferentiation of expanded chondrocytes and the concurrent generation of cartilaginous constructs. The demonstration of these synergistic effects also for adult human chondrocytes supports the clinical relevance of the findings. (C) 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN ARTICULAR CHONDROCYTES; IN-VITRO; MORPHOGENETIC PROTEINS; GENE-EXPRESSION; CARTILAGINOUS TISSUE; SKELETAL DEVELOPMENT; BETA-SUPERFAMILY; CROSS-TALK; CELLS; CULTURE; GDF-5; Cartilage; Tissue engineering; Redifferentiation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Hals-Nasen-Ohren-Heilkunde Medicine > Lehrstuhl für Orthopädie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Sep 2020 13:58 |
| Last Modified: | 02 Sep 2020 13:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28226 |
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