Wagner, Charlotte and Kurtz, Lisa and Schweda, Frank and Simon, Alexander M. and Kurtz, Armin (2009) Connexin 37 is dispensable for the control of the renin system and for positioning of renin-producing cells in the kidney. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 459 (1). pp. 151-158. ISSN 0031-6768,
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Within the juxtaglomerular apparatus, renin-producing cells and endothelial cells of the afferent arterioles express connexin (Cx)37 and Cx40, which form abundant gap junctions among these cells. Deletion of Cx40 leads to strong hyperreninemia and ectopic localization of renin-producing cells; however, the relevance of Cx37 for the renin system in the kidney has not been investigated. We therefore studied renin expression and renin secretion in kidneys from Cx37-deficient mice, both on normal salt diet and during chronic challenge of the renin system by pretreatment of mice with a low-salt diet in combination with an angiotensin I-converting enzyme inhibitor. This treatment procedure strongly enhances renin gene expression and renin secretion. We found that renal renin mRNA abundance and plasma renin concentration did not differ between wild-type and Cx37-/- mice under normal conditions. The stimulation of renin gene expression and renin secretion by salt depletion was even more pronounced in Cx37-/- as compared to wild-type mice. The regulation of renin secretion from isolated perfused kidneys by perfusion pressure and by angiotensin II was normal in Cx37-/- mice. In addition, the localization of renin-expressing cells was also regular in Cx37-/- kidneys. Finally, the expression pattern of other vascular Cxs such as Cx40, Cx43, and Cx45 was not altered in Cx37-/- kidneys. Our findings suggest that Cx37 is not essential for normal development and function of renin-producing cells. As a consequence, it appears unlikely that Cx40 exerts its important function in renin-producing cells via Cx37/Cx40 heteromeric gap junctions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RENAL VASCULATURE; ARTERIOLES; SECRETION; MICE; RAT; LOCALIZATION; CONDUCTION; EXPRESSION; JUNCTIONS; PRESSURE; Kidney; Renin; Gap junction; Renal perfusion pressure; Angiotensin |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 04:26 |
| Last Modified: | 03 Sep 2020 04:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28232 |
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