Soria, Rene Barro and Spitzner, Melanie and Schreiber, Rainer and Kunzelmann, Karl (2009) Bestrophin-1 Enables Ca2+-activated Cl- Conductance in Epithelia. JOURNAL OF BIOLOGICAL CHEMISTRY, 284 (43). pp. 29405-29412. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
Epithelial cells express calcium-activated Cl- channels of unknown molecular identity. These Cl- channels play a central role in diseases such as secretory diarrhea, polycystic kidney disease, and cystic fibrosis. The family of bestrophins has been suggested to form calcium-activated Cl- channels. Here, we demonstrate molecular and functional expression of bestrophin-1 (BEST1) in mouse and human airways, colon, and kidney. Endogenous calcium-activated whole cell Cl- currents coincide with endogenous expression of the Vmd2 gene product BEST1 in murine and human epithelial cells, whereas calcium-activated Cl- currents are absent in epithelial tissues lacking BEST1 expression. Blocking expression of BEST1 with short interfering RNA or applying an anti-BEST1 antibody to a patch pipette suppressed ATP-induced whole cell Cl- currents. Calcium-dependent Cl- currents were activated by ATP in HEK293 cells expressing BEST1. Thus, BEST1 may form the Ca2+-activated Cl- current, or it may be a component of a Cl- channel complex in epithelial tissues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED CHLORIDE CHANNELS; CYSTIC-FIBROSIS; MACULAR DYSTROPHY; PRIMARY CULTURES; PROTEIN-KINASE; HT(29) CELLS; CALCIUM; MOUSE; TRANSPORT; CURRENTS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 05:43 |
| Last Modified: | 03 Sep 2020 05:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28257 |
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