Funfak, Anette and Hartung, Regina and Cao, Jialan and Martin, Karin and Wiesmueller, Karl-Heinz and Wolfbeis, Otto S. and Koehler, J. Michael (2009) Highly resolved dose-response functions for drug-modulated bacteria cultivation obtained by fluorometric and photometric flow-through sensing in microsegmented flow. SENSORS AND ACTUATORS B-CHEMICAL, 142 (1). pp. 66-72. ISSN 0925-4005,
Full text not available from this repository. (Request a copy)Abstract
The application of the micro fluid segment technique for the investigation of highly resolved dose-response relationship at the physiological parameters segment-internal pH and growth of the cell culture is introduced. The method was used to study the concentration-dependent response of an Escherichia coli culture for the effectors 2,4-dinitrophenol (DNP) and an antibacterial peptide amide (KKVVFKVKFK-NH(2)). Large sequences with up to 250 micro fluid segments containing gradually varying concentrations of the effectors were generated using a PTFE micro fluidic arrangement, including a 7-port-manifold and PC-controlled syringe pumps. The response of the cell culture was characterized by a double sensor system allowing for simultaneous read out of metabolism-related changes as well as changes in cell density. A twin arrangement of a micro flow-through photometer and a micro flow-through fluorometer based on LED devices in connection with the application of pH-sensitive polymer sensor particles was applied. This experimental setup allows a detailed determination of drug-related changes in fluorescence intensity by the E. coli culture and the polymer particles as a function of time by tracking changes in pH and cell density. The application of the segmented flow technique for multi-parameter drug screenings provides new insights into the biological answer of bacteria cultures cultivated at the nanoliter scale. (c) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DARK-FIELD MICROSCOPY; SEGMENTED-FLOW; MICROFLUIDIC SYSTEM; DISSOLVED-OXYGEN; CHIP; PH; CHEMISTRY; PARAMETERS; DENSITY; BIOCHIP; Micro fluid segments; Droplet based flow; Antibiotics; Drug detection; Dose-response functions; On-line pH sensing; Cell density monitoring |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 08:11 |
| Last Modified: | 03 Sep 2020 08:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28278 |
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