Koos, Bjoern and Paulsson, Janna and Jarvius, Malin and Sanchez, Betzabe Chavez and Wrede, Brigitte and Mertsch, Sonja and Jeibmann, Astrid and Kruse, Anne and Peters, Ove and Wolff, Johannes E. A. and Galla, Hans-Joachim and Soderberg, Ola and Paulus, Werner and Ostman, Arne and Hasselblatt, Martin (2009) Platelet-Derived Growth Factor Receptor Expression and Activation in Choroid Plexus Tumors. AMERICAN JOURNAL OF PATHOLOGY, 175 (4). pp. 1631-1637. ISSN 0002-9440, 1525-2191
Full text not available from this repository. (Request a copy)Abstract
Choroid plexus tumors are intraventricular neoplasms predominantly affecting young children. in contrast to choroid plexus papillomas, choroid plexus carcinomas progress frequently, necessitating the development of adjuvant treatment concepts. Platelet derived growth factor (PDGF) signaling has been shown to support growth in a variety of tumors. The finding of PDGF receptor expression in choroid plexus tumors prompted us to elucidate PDGF receptor activation state using a novel method, in situ proximity ligation assay, on formalin-fixed, paraffin-embedded, archival samples of 19 choroid plexus tumors. As assessed by in situ proximity ligation assay, the proportion of phosphorylated PDGF receptor a was low in choroid plexus papillomas and choroid plexus carcinomas, whereas phosphorylated PDGF receptor 13 was found to be significantly higher in choroid plexus carcinomas. In the immortalized choroid plexus epithelial cell line Z310 expressing PDGF receptor 0, PDGF-BB exhibited a time- and dose-dependent proliferative response, which was significantly attenuated by imatinib (gleevec). In conclusion, our findings suggest that PDGF receptor 13 is functionally involved in the biology of choroid plexus tumors and may represent a molecular target for therapy. In addition, this study demonstrates the feasibility and usefulness of in situ proximity ligation assay for monitoring receptor tyrosine kinase activation in formalin-fixed, paraffin-embedded, archival tissues. (Am J Pathol 2009, 175:1631-1637; DOI: 10.2353/ajpath.2009.081022)
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMATINIB; BETA; TARGETS; ALPHA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Pädiatrische Hämatologie, Onkologie und Stammzelltransplantation |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 09:34 |
| Last Modified: | 03 Sep 2020 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28284 |
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