Lehle, Karla and Friedl, Lucas and Wilm, Julius and Philipp, Alois and Mueller, Thomas and Lubnow, Matthias and Schmid, Christof (2016) Accumulation of Multipotent Progenitor Cells on Polymethylpentene Membranes During Extracorporeal Membrane Oxygenation. ARTIFICIAL ORGANS, 40 (6). pp. 577-585. ISSN 0160-564X, 1525-1594
Full text not available from this repository. (Request a copy)Abstract
Multipotent progenitor cells were mobilized during pediatric extracorporeal membrane oxygenation (ECMO). We hypothesize that these cells also adhered onto polymethylpentene (PMP) fibers within the membrane oxygenator (MO) during adult ECMO support. Mononuclear cells were removed from the surface of explanted PMP-MOs (n=16). Endothelial-like outgrowth and mesenchymal-like cells were characterized by flow cytometric analysis using different surface markers. Spindle-shaped attaching cells were identified early, but without proliferative activity. After long-term cultivation palisading type or cobblestone-type outgrowth cells with high proliferative activity appeared and were characterized as (i) leukocytoid CD45+/CD31+ (CD133+/VEGFR-II+/CD90+/CD14+/CD146dim/CD105dim); (ii) endothelial-like CD45-/CD31+ (VEGF-RII+/CD146+/CD105+/CD133-/CD14-/CD90-); and (iii) mesenchymal-like cells CD45-/CD31- (CD105+/CD90+/CD133dim/VEGFR-II-/CD146-/CD14-). The distribution of the cell populations depended on the MO and cultivation time. Endothelial-like cells formed capillary-like structures and did uptake Dil-acetylated low-density lipoprotein. Endothelial- and mesenchymal-like cells adhered on the surface of PMP-MOs. Further research is needed to identify the clinical relevance of these cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE LUNG INJURY; UMBILICAL-CORD BLOOD; MULTIDETECTOR COMPUTED-TOMOGRAPHY; MESENCHYMAL STEM-CELLS; COLONY-FORMING CELLS; IN-VITRO; THROMBOTIC DEPOSITS; CARDIOVASCULAR RISK; ENDOTHELIAL-CELLS; BONE-MARROW; ECMO; Adherence; Endothelial-like cells; Mesenchymal stem cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2019 09:27 |
| Last Modified: | 22 Mar 2019 09:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2830 |
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