Jobst, Bertram J. and Riegger, Guenter A. J. and Griese, Daniel P. (2009) Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model. CARDIOVASCULAR DRUGS AND THERAPY, 23 (5). pp. 343-353. ISSN 0920-3206, 1573-7241
Full text not available from this repository. (Request a copy)Abstract
Aim of the study was to evaluate the therapeutic potential of endothelial cell seeding to inhibit the neointimal response after balloon injury of the rat carotid artery. Endothelial cells were isolated from peripheral blood after cytokine-stimulation (PB-EC), the bone marrow compartment (BM-EC), and the thoracic aorta (AO-EC) of male rats. The EC-populations were characterized by microscopy, cytofluorometry, and PCR-analysis, and displayed distinct patterns of RNA-expression of the EC-markers VEGF-receptor 1 and 2, and TIE2. 5 x 10(5) ECs were incubated in the freshly balloon-denuded arterial bed for 30 min achieving about 70% coverage with all 3 EC-populations. However, the neointimal response 2 weeks after the procedure was significantly aggravated in the EC-seeded carotids (I/M-ratio: non-seeded Control 1.00 +/- 0.10, PB-EC 1.53 +/- 0.07, BM-EC 1.64 +/- 0.12, and AO-EC 1.55 +/- 0.14; P < 0.05 vs. Control for all). We found evidence for an accelerated cell-turnover (TUNEL-assay, total cell-density, infiltration with CD45(pos) haematopoietic cells) especially in the adventitia of the treated vessels. Endothelial cell seeding fails to prevent intimal hyperplasia following arterial injury in the rat carotid model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; PROGENITOR CELLS; STEM-CELLS; TRANSPLANTATION; THERAPY; GRAFTS; RABBIT; ATHEROSCLEROSIS; INFLAMMATION; RESTENOSIS; Cell therapy; Endothelial cells; Seeding; Vascular injury; Rats; Adventitia |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Sep 2020 12:29 |
| Last Modified: | 03 Sep 2020 12:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28306 |
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