Molle, Julia and Raab, Mario and Holzmeister, Susanne and Schmitt-Monreal, Daniel and Grohmann, Dina and He, Zhike and Tinnefeld, Philip (2016) Superresolution microscopy with transient binding. CURRENT OPINION IN BIOTECHNOLOGY, 39. pp. 8-16. ISSN 0958-1669, 1879-0429
Full text not available from this repository. (Request a copy)Abstract
For single-molecule localization based superresolution, the concentration of fluorescent labels has to be thinned out. This is commonly achieved by photophysically or photochemically deactivating subsets of molecules. Alternatively, apparent switching of molecules can be achieved by transient binding of fluorescent labels. Here, a diffusing dye yields bright fluorescent spots when binding to the structure of interest. As the binding interaction is weak, the labeling is reversible and the dye ligand construct diffuses back into solution. This approach of achieving superresolution by transient binding (STB) is reviewed in this manuscript. Different realizations of STB are discussed and compared to other localization-based superresolution modalities. We propose the development of labeling strategies that will make STB a highly versatile tool for superresolution microscopy at highest resolution.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OPTICAL RECONSTRUCTION MICROSCOPY; DNA ORIGAMI; FLUORESCENCE MICROSCOPY; LIVING CELLS; SUBDIFFRACTION-RESOLUTION; LOCALIZATION MICROSCOPY; HIGH-DENSITY; PULL-DOWN; PROBES; STANDARDS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Dina Grohmann |
| Depositing User: | Petra Gürster |
| Date Deposited: | 02 Sep 2020 06:22 |
| Last Modified: | 02 Sep 2020 06:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2832 |
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