Echtenacher, B. and Wege, A. K. and Schardt, K. and Bitterer, F. and Gehmert, S. and Hoffmann, P. and Stroszczynski, C. and Jung, E. M. (2016) High-Resolution Ultrasound Including Contrast-Enhanced Ultrasound (CEUS) for the Detection of Gas Formation during Aspergillus Fumigatus Infection in Mice. ULTRASCHALL IN DER MEDIZIN, 37 (3). pp. 277-282. ISSN 0172-4614, 1438-8782
Full text not available from this repository. (Request a copy)Abstract
Purpose: A. fumigatus infections represent a major threat for patients with a suppressed immune system. Early diagnosis is of importance for a favorable outcome but appears to be difficult due to limited diagnostic procedures. Here we investigated the sensitivity of high-resolution ultrasound (HRU) for the detection of A. fumigatus infection in the liver. Materials and Methods: BALB/c mice were intravenously infected with A. fumigatus and monitored by HRU, Doppler sonography (CCDS), contrast-enhanced ultrasound (CEUS), and real-time strain color-coded elastography (CCE) using a multi-frequency probe (6 - 15 MHz). Contrast media bolus injection of sulfur-hexafluoride micro-bubbles was applied and digital cine-loops from the arterial phase, as well as the portal venous phase up to the late phase of the whole liver were analyzed. All data were correlated to the histopathological findings. Results: Using HRU and CEUS, a sonic shadow was detected in all infected animals. All Aspergillus-infected nodes from 3 -6mm in the liver showed a shadow with rim enhancement and no intranodal enhancement when using CEUS. A. fumigatus infection was confirmed by CFU assessment and histopathological analysis. Granulomas were not associated with shadowing on B-mode. In contrast, granulomas with a diameter above 5mm and a higher stiffness in CCE generated particularly an arterial rim enhancement and portal venous washout without contrast media uptake in the late phase. In addition, CEUS was able to define dynamic capillary microvascularization of infected liver areas. Conclusion: Liver lesions associated with A. fumigatus infection can be detected in mice when combined with CEUS and CCE in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FOCAL LIVER-LESIONS; INVASIVE PULMONARY ASPERGILLOSIS; SYSTEMIC FUNGAL-INFECTIONS; TRANSPLANT RECIPIENTS; MULTICENTER TRIAL; RISK-FACTORS; HYDROPHOBIN; SONOGRAPHY; DIAGNOSIS; CONIDIA; Aspergillus fumigatus; inflammation; real-time strain color-coded elastography (CCE); liver infection; contrast-enhanced ultrasound (CEUS) |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Pathologie Medicine > Lehrstuhl für Röntgendiagnostik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2019 09:39 |
| Last Modified: | 22 Mar 2019 09:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2837 |
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