Spiering, Desiree and Schmolke, Mirco and Ohnesorge, Nils and Schmidt, Marc and Goebeler, Matthias and Wegener, Joachim and Wixler, Viktor and Ludwig, Stephan (2009) MEK5/ERK5 Signaling Modulates Endothelial Cell Migration and Focal Contact Turnover. JOURNAL OF BIOLOGICAL CHEMISTRY, 284 (37). pp. 24972-24980. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
The formation of new blood vessels from pre-existing ones requires highly coordinated restructuring of endothelial cells (EC) and the surrounding extracellular matrix. Directed EC migration is a central step in this process and depends on cellular signaling cascades that initiate and control the structural rearrangements. On the basis of earlier findings that ERK5 deficiency in mouse EC results in massive defects in vessel architecture, we focused on the impact of the MEK5/ERK5 signaling pathway on EC migration. Using a retroviral gene transfer approach, we found that constitutive activation of MEK5/ERK5 signaling strongly inhibits EC migration and results in massive morphological changes. The area covered by spread EC was dramatically enlarged, accompanied by an increase in focal contacts and altered organization of actin filaments. Consequently, cells were more rigid and show reduced motility. This phenotype was most likely based on decreased focal contact turnover caused by reduced expression of p130Cas, a key player in directed cell migration. We demonstrate for the first time that ERK5 signaling not only is involved in EC survival and stress response but also controls migration and morphology of EC.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED PROTEIN-KINASE; NF-KAPPA-B; GENE-EXPRESSION; ADHESION KINASE; ERK5; MICE; P130(CAS); BMK1/ERK5; ACTIN; TRANSCRIPTION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Sep 2020 08:09 |
| Last Modified: | 07 Sep 2020 08:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28409 |
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