Panning, M. and Eickmann, M. and Landt, O. and Monazahian, M. and Oelschlaeger, S. and Baumgarte, S. and Reischl, U. and Wenzel, J. J. and Niller, H. H. and Guenther, S. and Hollmann, B. and Huzly, D. and Drexler, J. F. and Helmer, A. and Becker, S. and Matz, B. and Eis-Huebinger, A. M. and Drosten, Christian (2009) DETECTION OF INFLUENZA A(H1N1)V VIRUS BY REAL-TIME RT-PCR. EUROSURVEILLANCE, 14 (36): 19329. pp. 7-12. ISSN 1560-7917,
Full text not available from this repository. (Request a copy)Abstract
Influenza A(H1N1)v virus was first identified in April 2009. A novel real-time RT-PCR for influenza A(H1N1)v virus was set up ad hoc and validated following industry-standard criteria. The lower limit of detection of the assay was 384 copies of viral RNA per ml of viral transport medium (95% confidence interval: 273-876 RNA copies/ml). Specificity was 100% as assessed on a panel of reference samples including seasonal human influenza A virus H1N1 and H3N2, highly pathogenic avian influenza A virus H5N1 and porcine influenza A virus H1N1, H1N2 and H3N2 samples. The real-time RT-PCR assay for the influenza A matrix gene recommended in 2007 by the World Health Organization was modified to work under the same reaction conditions as the influenza A(H1N1)v virus-specific test. Both assays were equally sensitive. Clinical applicability of both assays was demonstrated by screening of almost 2,000 suspected influenza (H1N1)v specimens, which included samples from the first cases of pandemic H1N1 influenza imported to Germany. Measuring influenza A(H1N1)v virus concentrations in 144 laboratory-confirmed samples yielded a median of 4.6 log RNA copies/ml. The new methodology proved its principle and might assist public health laboratories in the upcoming influenza pandemic.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REVERSE TRANSCRIPTION-PCR; A H1N1 VIRUS; MOLECULAR-DETECTION; VIRAL LOAD; INFECTION; ASSAYS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Sep 2020 08:16 |
| Last Modified: | 07 Sep 2020 08:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28411 |
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