Stradalova, Vendula and Stahlschmidt, Wiebke and Grossmann, Guido and Blazikova, Michaela and Rachel, Reinhard and Tanner, Widmar and Malinsky, Jan (2009) Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1. JOURNAL OF CELL SCIENCE, 122 (16). pp. 2887-2894. ISSN 0021-9533, 1477-9137
Full text not available from this repository. (Request a copy)Abstract
Plasma membrane of the yeast Saccharomyces cerevisiae contains stable lateral domains. We have investigated the ultrastructure of one type of domain, the membrane compartment of Can1 (MCC). In two yeast strains (nce102 Delta and pil1 Delta) that are defective in segregation of MCC-specific proteins, we found the plasma membrane to be devoid of the characteristic furrow-like invaginations. These are highly conserved plasma membrane structures reported in early freeze-fracture studies. Comparison of the results obtained by three different approaches - electron microscopy of freeze-etched cells, confocal microscopy of intact cells and computer simulation - shows that the number of invaginations corresponds to the number of MCC patches in the membrane of wild-type cells. In addition, neither MCC patches nor the furrow-like invaginations colocalized with the cortical ER. In mutants exhibiting elongated MCC patches, there are elongated invaginations of the appropriate size and frequency. Using various approaches of immunoelectron microscopy, the MCC protein Sur7, as well as the eisosome marker Pil1, have been detected at these invaginations. Thus, we identify the MCC patch, which is a lateral membrane domain of specific composition and function, with a specific structure in the yeast plasma membrane - the furrow-like invagination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; FREEZE-FRACTURE; REGENERATING PROTOPLASTS; ELECTRON-MICROSCOPY; CANDIDA-ALBICANS; FINE-STRUCTURE; PROTEIN; CELLS; ULTRASTRUCTURE; DOMAINS; Eisosome; Electron microscopy; MCC; Membrane compartmentalization; Plasma membrane invagination |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Sep 2020 09:31 |
| Last Modified: | 09 Sep 2020 09:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28566 |
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