Schwarz, Timo and Seidl, Christof and Schiemann, Matthias and Senekowitsch-Schmidtke, Reingard and Krause, Bernd Joachim (2016) Increased choline uptake in macrophages and prostate cancer cells does not allow for differentiation between benign and malignant prostate pathologies. NUCLEAR MEDICINE AND BIOLOGY, 43 (6). pp. 355-359. ISSN 0969-8051, 1872-9614
Full text not available from this repository. (Request a copy)Abstract
Introduction: Inflammatory cells may contribute to the choline uptake in different prostate pathologies. The aim of this study was (i) to assess if inflammatory cells incorporate choline and (ii) to potentially detect differences compared to FDG uptake. Therefore we investigated the uptake of [H-3]choline and [F-18]FDG in human prostate carcinoma cells and human inflammatory cells. Methods: Macrophages were cultured from isolated mononuclear cells, gained by density gradient centrifugation of human buffy coats. T-lymphocytes, B-lymphocytes and granulocytes were enriched by density gradient centrifugation before cell sorting by means of flow cytometry was performed. [H-3]choline and [F-18]FDG uptake of isolated inflammatory cells as well as of LNCaP and PC-3 human prostate carcinoma cells was assessed simultaneously in dual tracer uptake experiments. Results: Macrophages showed highest [H-3]choline and [F-18]FDG uptake compared to the tracer uptake rates of leukocytes. [H-3]choline uptake of macrophages was in the same range as in prostate cancer cells. Lipopolysaccharide stimulation of macrophages resulted in an increase of [F-18]FDG uptake in macrophages, but not in an increased [H-3]choline uptake. Conclusions: The high [H-3]choline uptake in macrophages may be a source of false-positive PET results in diagnosis of prostate cancer by choline-PET/CT. As already known from FDG-PET, discrimination between tumor and inflammation in prostate cancer patients is not possible via choline-PET. Advances in knowledge and implications for patient care: The application of choline-PET for reliable primary prostate cancer detection and delineation has to be queried. (C) 2016 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POSITRON-EMISSION-TOMOGRAPHY; SOFT-TISSUE INFECTION; C-11-CHOLINE PET; LYMPHOCYTE-ACTIVATION; HIGH ACCUMULATION; F-18-FDG PET; INFLAMMATION; TUMOR; FDG; FLUORINE-18-FLUORODEOXYGLUCOSE; [3H]choline; [18F]FDG; PC-3 cells; LNCaP cells; Leucocytes; Inflammatory cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Mar 2019 09:55 |
| Last Modified: | 22 Mar 2019 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2857 |
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