Lieb, S. and Michaelis, S. and Plank, N. and Bernhardt, G. and Buschauer, A. and Wegener, J. (2016) Label-free analysis of GPCR-stimulation: The critical impact of cell adhesion. PHARMACOLOGICAL RESEARCH, 108. pp. 65-74. ISSN 1043-6618,
Full text not available from this repository. (Request a copy)Abstract
Label-free cell-based assays have been attracting growing attention in drug research. Optical approaches based on evanescent electric fields (e.g. EPIC, RWG/DMR, SPR) and electrochemical impedance analysis (ECIS, xCELLigence) are by far the most widespread techniques for such purposes. We compared three label-free approaches (ECIS, RWG/DMR and SPR) with respect to the activation of the human histamine H-1 receptor (H1R) expressed by U-373 MG glioblastoma and genetically engineered HEK 293T cells. HEK 293T cells were either expressing the hH(1)R alone or in combination with the adhesion protein hMSR1. The beta(2)-adrenergic receptor (beta(2)-AR) expressed by bovine aortic endothelial cells (BAEC) served as a second cell model. Reduced cell adhesion to the surface of the sensing devices affected both, the optical and the impedance-based readout, but became much more obvious in case of RWG- or SPR-based assays. By contrast, the co-expression of hH(1)R and hMSR1 in HEK 293T cells strongly enhanced the signal compared to hH(1)R expression alone. As the sensitivity of the optical readouts is confined to a distance of 100-200 nm from the surface, depending on the wavelength of the incident light, this observation is in accordance with tighter adhesion of the co-transfectants, inducing a shorter distance between the cell membrane and the substrate. Combining ECIS and SPR, allowing for simultaneous registration of both signals for a single cell population, provided a direct correlation of both readouts, when H1R or (beta(2)-AR stimulation was investigated for the same cell populations. Cell adhesion was found to have a critical impact on the results of label-free cell monitoring, in particular when techniques based on evanescent electric fields are applied. (C) 2016 Published by Elsevier Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTORS; SCAVENGER RECEPTOR; DRUG DISCOVERY; CULTURE; ASSAYS; Label-free; Cell-based assays; G-protein-coupled receptors; Holistic; ECIS; SPR; Dynamic mass redistribution (DMR) |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2019 14:28 |
| Last Modified: | 15 Mar 2019 12:05 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2858 |
Actions (login required)
 |
View Item |
