Sporrer, D. and Weber, M. and Wanninger, J. and Weigert, J. and Neumeier, M. and Stoegbauer, F. and Lieberer, E. and Bala, M. and Kopp, A. and Schaeffler, A. and Buechler, C. (2009) Adiponectin downregulates CD163 whose cellular and soluble forms are elevated in obesity. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 39 (8). pp. 671-679. ISSN 0014-2972, 1365-2362
Full text not available from this repository. (Request a copy)Abstract
P>Background CD163 is a monocyte/macrophage specific receptor whose soluble form (sCD163) is elevated in inflammatory diseases. Obesity is associated with chronic inflammation and low adiponectin, an anti-inflammatory adipokine. Adiponectin, 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR) and metformin activate the AMP-kinase that exerts anti-inflammatory effects, and the influence of adiponectin and these drugs on monocytic CD163 was analysed, and cellular and sCD163 were determined in obesity and type 2 diabetes. Materials and methods Monocytes were incubated with adiponectin, AICAR or metformin. Furthermore, monocytes and serum were obtained from type 2 diabetic patients (T2D), overweight (defined as a body mass index >= 25 kg m(-2)) and normal-weight (NW) controls. CD163 was analysed by immunoblot and sCD163 was measured by enzyme-linked immunosorbent assay in the supernatants of the monocytes and in serum. Results In monocytes, adiponectin reduced cellular and surface CD163, whereas sCD163 was not altered in the corresponding supernatants. Further, metformin and AICAR downregulated CD163. Monocytic CD163 was higher in T2D and obesity, whereas sCD163 in the supernatants was not elevated and neither correlated with serum sCD163 nor systemic adiponectin. There was a positive correlation of monocytic sCD163 with serum but not with monocytic IL-6. In the serum of obese controls and T2D patients, sCD163 was significantly higher compared to NW donors and was positively associated with systemic IL-6. Conclusions This study indicates that monocytic CD163 and systemic sCD163 are elevated in T2D and obesity. Adiponectin reduces CD163 in vitro, but additional factors related to obesity like IL-6 may be more relevant in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEMOGLOBIN SCAVENGER RECEPTOR; ACTIVATED PROTEIN-KINASE; TOLL-LIKE RECEPTORS; ADIPOSE-TISSUE; INSULIN-RESISTANCE; IL-6 PRODUCTION; HUMAN MONOCYTES; DISEASE; MACROPHAGES; AMPK; Adiponectin; CD163; monocyte; type 2 diabetes mellitus |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Sep 2020 07:27 |
| Last Modified: | 10 Sep 2020 07:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28614 |
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