Winklmeier, Andreas and Weyand, Michael and Schreier, Christina and Kalbitzer, Hans Robert and Kremer, Werner (2009) Crystallization and preliminary X-ray diffraction studies of the tetramerization domain derived from the human potassium channel Kv1.3. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 65. pp. 688-691. ISSN 2053-230X,
Full text not available from this repository. (Request a copy)Abstract
The tetramerization domain (T1 domain) derived from the voltage-dependent potassium channel Kv1.3 of Homo sapiens was recombinantly expressed in Escherichia coli and purified. The crystals were first grown in an NMR tube in 150 mM potassium phosphate pH 6.5 in the absence of additional precipitants. The crystals showed I4 symmetry characteristic of the naturally occurring tetrameric assembly of the single subunits. A complete native data set was collected to 1.2 angstrom resolution at 100 K using synchrotron radiation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT K+ CHANNEL; CRYSTAL-STRUCTURE; 3-DIMENSIONAL STRUCTURE; CONFORMATIONAL-CHANGES; T1 DOMAIN; INACTIVATION; MEMBRANE; SUBUNIT; STATE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Sep 2020 10:37 |
| Last Modified: | 10 Sep 2020 10:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28713 |
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