Lehle, Karla and Stock, Martin and Schmid, Thomas and Schopka, Simon and Straub, Rainer H. and Schmid, Christof (2009) Cell-Type Specific Evaluation of Biocompatibility of Commercially Available Polyurethanes. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 90B (1). pp. 312-318. ISSN 1552-4973,
Full text not available from this repository. (Request a copy)Abstract
The biocompatibility of different commercially available poly (ether) urethane (PUR), medically used as main component for pump chambers of implantable ventricular assist devices (VAD), was evaluated. We investigated the influence of the PUR manufacturing process in an in vitro cytotoxicity screening assay. Human saphenous vein endothelial cells (HSVEC) and a Mouse fibroblast cell line (L929) were cultivated with different PUR specimens. Tissue-cultured polystyrole (TCP) was used as a reference. The cytotoxic effect was evaluated by morphology (phase contrast microscopy), cell viability (mitochondrial acitvity), cell growth kinetics, and proliferation (incorporation of (3)H-methyl-thymidine) tests. Fibronectin-coating guaranteed the adhesion of both cell types onto the reference material. Sterilization procedure of test materials did not affect adhesion properties. L929 completely covered the surfaces of Tecothane (R), Carbothane (R), and Mecora specimens, whereas HSVEC formed an imperfect monolayer onto the PUR. The mitochondrial activity was reduced in all cell types attached to PUR. In addition, proliferation of cells was not observed when using these materials. Commercially available PUR provided an unfavorable support for colonization of patient-derived HSVEC, which demanded a surface modification. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 90B: 312-318, 2009
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN VASCULAR CELLS; ENDOTHELIAL-CELLS; ASSIST DEVICE; IN-VITRO; ADHESION; CYTOTOXICITY; FIBROBLASTS; EXPRESSION; MEMBRANE; SURFACES; patient-derived endothelial cells; cell adhesion; mitochondrial activity; proliferation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2020 04:48 |
| Last Modified: | 14 Sep 2020 04:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28757 |
Actions (login required)
 |
View Item |
