A Fluorescent Probe for Diacetyl Detection

Li, Xiaohua and Duerkop, Axel and Wolfbeis, Otto S. (2009) A Fluorescent Probe for Diacetyl Detection. JOURNAL OF FLUORESCENCE, 19 (4). pp. 601-606. ISSN 1053-0509, 1573-4994

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Abstract

A water-soluble fluorescent probe, rhodamine B hydrazide (RBH), was prepared and its properties for recognition of diacetyl were studied. The method employs the reaction of diacetyl with RBH, a colorless and non-fluorescent rhodamine B spiro form derivative to give a pink-colored fluorescent substance. In weakly acidic media, RBH reacts more selectively with diacetyl than with other carbonyls, causing a large increase in fluorescence intensity and thereby providing an easy assay for the determination of diacetyl.

Item Type: Article
Uncontrolled Keywords: LIQUID-CHROMATOGRAPHY; ACETOIN; CULTURE; BUTTER; MILK; Fluorescent probe; Rhodamine B hydrazide; Diacetyl; Cell medium
Subjects: 500 Science > 540 Chemistry & allied sciences
Divisions: Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 14 Sep 2020 05:15
Last Modified: 14 Sep 2020 05:15
URI: https://pred.uni-regensburg.de/id/eprint/28760

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