Matysik, Silke and Matysik, Frank-Michael (2009) Microextraction by packed sorbent coupled with gas chromatography-mass spectrometry: application to the determination of metabolites of monoterpenes in small volumes of human urine. MICROCHIMICA ACTA, 166 (1-2). pp. 109-114. ISSN 0026-3672,
Full text not available from this repository. (Request a copy)Abstract
A miniaturized sample preparation method is presented that is based on microextraction by packed sorbent (MEPS) to determine metabolites of monoterpenes in small volumes of human urine. A protocol including syringe modifications has been developed to exploit the concept of MEPS for application in gas chromatography-mass spectrometry with splitless injection. Only 210 mu L of enzymatically hydrolysed urine is needed to carry out a complete extraction protocol. Elution volumes between 1 and 10 mu L can be transferred completely or partly to the GC-MS system. All steps of filling and injection of the MEPS syringes are performed automatically. The utility of this approach was demonstrated in terms of determinations of the monoterpene metabolites cis-verbenol, trans-verbenol, alpha-terpineol, myrtenol and perillyl alcohol in a urine sample of a volunteer who lived in a dwelling with high indoor air exposure of monoterpenes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STRONG AIRWAY IRRITANTS; OCCUPATIONAL-EXPOSURE; SYRINGE; SAMPLES; ONLINE; IDENTIFICATION; TERPENES; MIXTURE; LIQUID; WATER; Microextraction by packed sorbent; Monoterpenes; Biomonitoring |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2020 05:56 |
| Last Modified: | 14 Sep 2020 05:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28779 |
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