Tyagi, A. and Penzkofer, Alfons and Batschauer, A. and Wolf, E. (2009) Fluorescence behaviour of 5,10-methenyltetrahydrofolate, 10-formyltetrahydrofolate, 10-formyldihydrofolate, and 10-formylfolate in aqueous solution at pH 8. CHEMICAL PHYSICS, 361 (1-2). pp. 75-82. ISSN 0301-0104, 1873-4421
Full text not available from this repository. (Request a copy)Abstract
The fluorescence spectroscopic behaviour of (6R,S)-5,10-methenyltetrahydrofolate (MTHF), (6R,S)-10-formyltetrahydrofolate (10-HCO-H4folate), 10-formyldihydrofolate (10-HCO-H2folate), and 10-formylfolate (10-HCO-folate) in aqueous Tris-HCl buffer at pH 8 is studied. MTHF and 10-HCO-folate were commercially available. 10-HCO-H4folate was prepared from MTHF by hydrolysis at room temperature under anaerobic conditions. 10-HCO-H2folate was prepared by oxidation of 10-HCO-H4folate under aerobic conditions. Fluorescence quantum distributions at room temperature and fluorescence signal decays at room temperature and liquid nitrogen temperature were measured. The fluorescence lifetimes determined at room temperature (liquid nitrogen temperature) are 10 ps (2.9 ns) for MTHF, 38 ps (3.7 ns) for 10-HCO-H4folate, 80 ps (10.5 ns) for 10-HCO-H2folate, and 7.1 ns (20 ns) for 10-HCO-folate. The results are discussed in terms of dyadic (pterin-benzoyl-glutamate) photo-induced electron transfer and dyadic fluorescent dynamics. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLUE-LIGHT PHOTORECEPTORS; COLI DNA PHOTOLYASE; ESCHERICHIA-COLI; SPECTROSCOPIC CHARACTERIZATION; FOLIC-ACID; ARABIDOPSIS-THALIANA; PTERIN DERIVATIVES; ABSORPTION; TETRAHYDROFOLATE; IDENTIFICATION; (6R,S)-5,10-Methenyltetrahydrofolate chloride (MTHF-Cl); (6R,S)-10-Formyltetrahydrofolate (10-HCO-H4folate); 10-Formyldihydrofolate (10-HCO-H2folate); 10-Formylfolate (10-HCO-folate); Fluorescence quantum yield; Fluorescence quantum distribution; Fluorescence lifetime; Photo-induced charge transfer; Dyadic behaviour |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2020 06:56 |
| Last Modified: | 14 Sep 2020 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28811 |
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