Gruenewald, Christoph and Botella, Jose A. and Bayersdorfer, Florian and Navarro, Juan A. and Schneuwly, Stephan (2009) Hyperoxia-induced neurodegeneration as a tool to identify neuroprotective genes in Drosophila melanogaster. FREE RADICAL BIOLOGY AND MEDICINE, 46 (12). pp. 1668-1676. ISSN 0891-5849, 1873-4596
Full text not available from this repository. (Request a copy)Abstract
Oxidative stress has been reported to be a common underlying mechanism in the pathogenesis of many neurodegenerative disorders such as Alzheimer, Huntington, Creutzfeld-Jakob, and Parkinson disease. Despite the increasing number of articles showing a correlation between oxidative damage and neurodegeneration little is known about the genetic elements that confer protection against the deleterious effects of an oxidative imbalance in neurons. We show that oxygen-induced damage is a direct cause of brain degeneration in Drosophila and establish an experimental setup measuring dopaminergic neuron survival to model oxidative stress-induced neurodegeneration in flies. The overexpression of superoxide dismutase but not catalase was able to protect dopaminergic neurons against oxidative imbalance under hyperoxia treatment. In an effort to identify new genes involved in the process of oxidative stress-induced neurodegeneration, we have carried out a genome-wide expression analysis to identify genes whose expression is upregulated in fly heads under hyperoxia. Among them, a number of mitochondrial and cytoplasmic chaperones could be identified and were shown to protect dopaminergic neurons when overexpressed, thus validating our approach to identifying new genes involved in the neuronal defense mechanism against oxidative stress. (C) 2009 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OXIDATIVE STRESS; PARKINSONS-DISEASE; LIFE-SPAN; SUPEROXIDE-DISMUTASE; TYROSINE-HYDROXYLASE; EXPRESSION; OVEREXPRESSION; CATALASE; RESISTANCE; NEURONS; Drosophila; Oxidative stress; Neurodegeneration; Hyperoxia; Genome-wide expression; SOD; Catalase; Chaperone; Hsp70; Hsc70-4; Hsc70-3; Hsp22; Free radicals |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2020 07:06 |
| Last Modified: | 14 Sep 2020 07:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28820 |
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