Wolf, Michael and Lossdoerfer, Stefan and Roemer, Piero and Kirschneck, Christian and Kuepper, Katharina and Deschner, James and Jaeger, Andreas (2016) Short-term heat pre-treatment modulates the release of HMGB1 and pro-inflammatory cytokines in hPDL cells following mechanical loading and affects monocyte behavior. CLINICAL ORAL INVESTIGATIONS, 20 (5). pp. 923-931. ISSN 1432-6981, 1436-3771
Full text not available from this repository. (Request a copy)Abstract
Heat shock proteins (HSP) act as cell-protective molecules that are upregulated upon thermal insult, hypoxia, and ischemia. Such ischemic conditions can be found during tissue remodeling associated with orthodontic tooth movement or trauma when compression forces lead to cell necrosis and subsequent clearance of cellular debris by immune competent cells. Host immune overreaction can result in undesired side effects such as tooth root resorption. Here, we analyzed whether heat pre-treatment would affect the initially catabolic host immune response induced by mechanical loading of human periodontal ligament (hPDL) cells, which represent major constituents of the tooth supporting apparatus involved in the regulation of periodontal remodeling. Fifth passage hPDL cells were exposed to an elevated temperature of 43A degrees for 1 h prior to mechanical loading. Cell morphology, high mobility group box protein 1 (HMGB1), interleukin (IL)-6, and IL-8 expression were analyzed microscopically and by ELISA. The physiological relevance for monocyte behavior was tested in monocyte adhesion and osteoclast differentiation assays. Short-term heat pre-treatment did not show any visible effect on hPDL cell morphology, but resulted in a significant downregulation of pro-inflammatory cytokines when being additionally loaded mechanically. Supernatants of heat-exposed hPDL cell cultures demonstrated a reduced impact on monocyte adhesion and osteoclastic differentiation. Heat pre-treatment of hPDL cells induces cell-protective mechanisms towards mechanical stress and favors the reduction of cell stress associated effects on monocyte/macrophage physiology. These data present the induction of heat shock proteins as a promising treatment option to limit undesired side effects of periodontal remodeling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PERIODONTAL-LIGAMENT CELLS; GROUP BOX PROTEIN-1; ORTHODONTIC TOOTH MOVEMENT; HUMAN TENDON FIBROBLASTS; NF-KAPPA-B; IN-VITRO; SHOCK PROTEINS; RANKL EXPRESSION; TENSION SIDES; UP-REGULATION; Heat shock proteins; Mechanical loading; Human PDL cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Mar 2019 13:46 |
| Last Modified: | 25 Mar 2019 13:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/2888 |
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