Schuetz, Christian and Fischer, Karin and Voelkl, Simon and Hoves, Sabine and Halbritter, Dagmar and Mackensen, Andreas and Fleck, Martin (2009) A new flow cytometric assay for the simultaneous analysis of antigen-specific elimination of T cells in heterogenous T cell populations. JOURNAL OF IMMUNOLOGICAL METHODS, 344 (2). pp. 98-108. ISSN 0022-1759,
Full text not available from this repository. (Request a copy)Abstract
Novel immunosuppressive strategies are targeting for an antigen-specific deletion of T cells responsible for Organ damage in autoimmunity and allograft rejection. Here, we present a new flow cytometry-based assay that allows the reliable and efficient detection of T cells that were eliminated in an antigen-specific fashion. A stable cell-labelling technique utilizing the two membrane dyes PKH26 and PKH67 has been combined with annexin V and 7-aminoactinomycin (7-AAD) staining to detect apoptotic cells. A differential gating strategy enabled us to determine the viability/apoptosis for each PKH-stained T cell subpopulation independently The capability to simultaneously analyze apoptosis within T cell mixtures of different antigen specificities establishes this assay as a superior tool for the further development of novel antigen-specific immunosuppressive approaches. (C) 2009 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LYMPHOCYTE ACTIVITY; ANNEXIN-V; PRESENTING CELLS; CYTOTOXICITY; APOPTOSIS; PHOSPHATIDYLSERINE; FLUOROMETRY; MODEL; VIVO; Flow cytometry; PKH26; PKH67; Cytotoxic T lymphocytes; Apoptosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Sep 2020 10:49 |
| Last Modified: | 15 Sep 2020 10:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/28956 |
Actions (login required)
 |
View Item |
