Antoine, Marianne and Koehl, Roman and Tag, Carmen G. and Gressner, Axel M. and Hellerbrand, Claus and Kiefer, Paul (2009) Secreted cysteine-rich FGF receptor derives from posttranslational processing by furin-like prohormone convertases. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 382 (2). pp. 359-364. ISSN 0006-291X, 1090-2104
Full text not available from this repository. (Request a copy)Abstract
Cysteine-rich FGF receptor (CFR) was originally identified as a FGF2 receptor and found to be identical to Golgi complex-localized glycoprotein-1 (GLG1), also known as MG-160, and to a murine E-selectin ligand-1 (ESL-1). Although CFR is a 150-kDa integral membrane glycoprotein that is primarily located in the cis-medial Golgi complex, a substantial proportion of CFR is secreted but the underlying mechanism is unknown. CFR contains several possible furin-like proprotein convertase (PC) and matrix metalloproteinase cleavage sites. Cells expressing CFR were treated with the furin protease inhibitor decanoyl-Arg-Val-Lys-Arg-chloromethylketone (decCMK) or the MMP-inhibitor GM6001. In the presence of furin-like PC inhibitor, secretion of CFR was almost completely inhibited. Secretion was not affected by the GM6001 inhibitor. The secreted forms were further characterized by creating different mutant CFR proteins with N-terminal and C-terminal tags. Immunoblot analysis and immunofluorescence indicated. that successive endoproteolytical processing of CFR which takes place in the Golgi complex is essential for secretion. Secreted CFR bound to heparan sulphate proteoglycan (HSPG) could trap FGFs and thereby directly competing with tyrosine kinase receptors for FGF binding. (C) 2009 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FIBROBLAST-GROWTH-FACTOR; SELECTIN-LIGAND ESL-1; PROPROTEIN CONVERTASES; GOLGI; ACTIVATION; INHIBITORS; CELLS; Cysteine-rich fibroblast growth factor receptor (CFR); Fibroblast growth factor (FGF); E-selectin ligand-1 (ESL-1); Furin; Proprotein convertase (PC); GLG1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2020 05:08 |
| Last Modified: | 16 Sep 2020 05:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/29002 |
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